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KMID : 0613820070170091294
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Journal of Life Science
2007 Volume.17 No. 9 p.1294 ~ p.1297
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Study of Distance Relationships among Domestic Radish (Raphanus sativus L.) by Analyzing its Anti-fungal Protein Gene
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Hwang Cher-Won
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Abstract
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To define diversity of domestic radish, we analysis genetic relationship of anti-fungal protein genes from several domestic radish (Raphanus sativus L.) seeds. We have isolated from domestic radish (Baekwoon) anti-fungal protein named RAP[12]. In this report, we isolate RNAs and raw protein from radish seeds then, RT-PCR analysis was done with another known anti-fungal sequences of radish from Gene Bank/EMBL and anti-fungal, anti-yeast activity were done against Botrytis cenerea , Saccharomyces cerevisiae, Candida albicans with it’s raw proteins. The anti-fungal activity was shown used all seeds but anti-yeast activity was shown only two seeds (Myungsan, Baekwoon). RT-PCR products (about 0.2 Kb) were not shown only two seeds. To identify the sequencing relationship of the domestic radish, we have cloned and sequenced RAP genes of the radish and analysis the sequence relationship with clustalw program. Thus we report the result that there are some different relationship between domestic radish and known other radish’s anti- fungal protein[15].
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KEYWORD
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Radish (Raphanus sativus L.), anti-microbial protein, distance relationship
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